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Pedro Goncalves

Dr. Pedro Goncalves

Experienced Researcher / Post-doc

Institut National De La Sante Et De La Recherche
Medicale (INSERM), France

August 2013 - July 2015

Biography

2008-2012: PhD in Metabolism, Clinical and Experimentation - Faculty of Medicine, University of Porto (FMUP), PT PhD thesis - "Transport of butyric acid in the epithelium of the colon – repercussions in colorectal cancer".
2006: Postgraduate course of the Master in Medicine and Molecular Oncology. Faculty of Medicine, University of Porto (FMUP), PT.
2000-2005: Degree in Applied Biology - (14/20 values), School of Sciences, University of Minho (UM), Braga, PT.
Area of research: Applied Biochemistry. Molecular Biology. Pharmacology. Oncobiology. Metabolism. Immunology.
Actual Scientific Interests: Wound healing in diabetes. Role of B and T lymphocytes in diabetes and wound healing.

QuanTI research project

TCR repertoire of CD8+ T cells in murine lymphocytic choriomeningitis virus infection

Many characteristics of persistent viral infections in humans (e.g. HIV, HCV, or HBV) are also observed during chronic infection of mice with lymphocytic choriomeningitis virus (LCMV). For example, infection with LCMV is associated with a progressive functional impairment and even leads to deletion of the virus-specific CD8+ T cell response. CD8+ T cells play an important role in adaptive immunity against virus-infected cells and tumor cells. Therefore, advancing our understanding of the CD8+ T cell responses may play an important role in controlling infections has important implications for the design of CD8 T cell epitope-based vaccines. The number of unique TCRαβ pairs in naïve T cells is estimated to about 2× 106 in mice. A TCR repertoire is considered more diverse if it contains more V(D)J combinations. Interestingly, studies suggets a compelling evidence of a link between TCR diversity and protection from infection (Messaoudi et al., 2002). TCR repertoires responding to a particular antigenic epitope are distinct between individuals. However, certain viral epitopes have also been shown to recruit preferentially a restricted TCR repertoire and some observations suggests public TCRs in a variety of infectious diseases. Understanding how CD8+ T cells repertoires are selected during differentiation and upon antigenic challenge is important at a therapeutic level for vaccine design and immunotherapy. In this study we analyze the repertoires of CD8+ T cells following LCMV infection. Naïve CD8+ T cells specific for from lymph nodes, spleen and bone marrow were identified directly ex vivo with cognate fluorescent pMHCI tetramers for LCMV epitope GP33 (Price et al., 2005) and then sorted by flow cytometry for TRBV13. Following, deep sequencing by multiplex single-nested PCR and direct sequencing of PCR products.

Supervisors

Rocha Benedita, M. D., Ph.D.; Directeur INSERM U1020 Necker Institut, Medical Faculty René Descartes Paris 5

Publications

Gonçalves P, Martel F. Butyrate and colorectal cancer: the role of BT transport. Curr Drug Metab. 2013;14(9):994-1008;

Gonçalves P, Gregório I, Catarino T, Martel F. The effect of oxidative stress upon the intestinal epithelial uptake of butyrate. Eur J Pharmacol. 2013; 29;699(1-3):88-100;

Gonçalves P, Catarino T, Gregório I, Martel F. Inhibition of butyrate uptake by the primary bile salt chenodeoxycholic acid in intestinal epithelial cells. J Cell Biochem. 2012;113(9):2937-47;

Gonçalves P, Gregório I, Araújo JR, Martel F. The effect of clotrimazole on energy substrate uptake and carcinogenesis in intestinal epithelial cells. Anticancer Drugs. 2012;23(2):220-9;

Gonçalves P, Gregório I, Martel F. The short-chain fatty acid butyrate is a substrate of breast cancer resistance protein. Am J Physiol Cell Physiol. 2011;301(5):C984-94;

Gonçalves P, Araújo JR, Martel F. Characterization of butyrate uptake by non-transformed intestinal epithelial cell lines. Journal of Membrane Biology 2011;240(1):35-46;

Gonçalves P., Araújo J.R., Pinho MJ., Martel F. In vitro studies on the inhibition of colon cancer by butyrate and polyphenolic compounds. Nutrition and Cancer. 2011;63(2):282-94;

Gonçalves P, Araújo J.R., Pinho M.J., Martel F. Modulation of butyrate transport in Caco-2 cells. Naunyn Schmiedebergs Arch Pharmacol 2009;379(4):325-36;

Gonçalves P., Araújo J.R., Martel F. The effect of high glucose upon SERT, the human plasmalemmal serotonin transporter. Nutritional Neuroscience 2008;11(6):244-50;

Gonçalves P., Araújo J.R., Azevedo I., Martel F. Lack of significant effect of cannabinoids upon the uptake of 2-deoxy-D-glucose by Caco-2 cells. Pharmacology. 2008;82(1):30-7.

 




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Associated partners
Universidade de Vigo

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Last update: 11 June 2017