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Melania Barile

Melania Barile

Early Stage Researcher / PhD student

Deutsches Krebsforschungszentrum (DKFZ, German Cancer Research Centre)
Theoretical System Biology
Im Neuenheimer Feld 267, 69120 Heidelberg
Germany

October 2013 - October 2016

Biography

Master degree in Physics (2009-2012), Bachelor degree in Physics (2006-2009), both at the University of Naples "Federico II". My master thesis title is "Models of complex regulation of the Myod1 gene", where I worked on a thermodynamic model to predict gene’s expression levels.

Scientific interests: Modelling of complex systems, especially biological ones, programming, statistics.

Hobbys: books, travels, sport

QuanTI research project

Mathematical Modeling of Hematopoiesis

The main topic of my project is the quantitative investigation of the machinery underlying hematopoiesis. The work is organized in two parts. The first issue we address is the comparison of different models describing the process of hematopoietic stem cells differentiating into all the mature hematopoietic cells. Especially we focus on the classically accepted model, according to which the two main lineages of hematopoietic cells, the myeloid and the lymphoid lineages, arise from a common predecessor, the multi-potent progenitor, which in turn descends directly from the very first stages of development, the long and the short term stem cells. While testing models' validity we also address the quantification of the parameters characterizing the stem cells developmental path, that are: proliferation rate for each sub-compartment, differentiation rate, death rate. Those analysis are performed fitting the experimental data yielded by an experiment in which stem cells were labeled in situ. The tools are both stochastic simulations and deterministic modeling. The second aspect is to focus on a particular stage along the myeloid lineage, the so called Colony-forming unit-erythroid (CFU-E). The intent is to analyze more peculiar aspects concerning differentiation, that are:
- determining the correct order according to which the different CFU-E stages arise
- investigating the influence of Epo on cells' proliferation and differentiation
- searching for the transcription factors involved in the processes This again is to be done by means of both stochastic simulations and deterministic modeling.

Figure 1
Figure 1. Classical scheme for Hematopoiesis

Figure 2
Figure 2. Fit of the available data by means of deterministic model

Supervisors

Professor Doctor Thomas Höfer, Deutsches Krebsforschungszentrum




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Funded by the EU
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Associated partners
Universidade de Vigo

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Last update: 11 June 2017