[c]
Registration: free
To register, please email i2m  maths.leeds.ac.uk
Information
Programme
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09:30
Carmen Gerlach (Netherlands Cancer Institute)
Mapping T cell fate at the clonal level by cellular barcoding
CD8+ T cell responses to infection are highly heterogeneous with
respect to surface marker expression, cytokine production and
longevity of the responding T cells. Nevertheless it is largely
unclear how these distinct subsets arise. To investigate how
heterogeneity within the CD8+ T cell lineage is generated, we have
developed a novel technology - called cellular barcoding - that
enables fate mapping of individual naive T cells. This technology is
based on the introduction of unique genetic tags into single naive T
cells that transmit their specific tag to all progeny. Thereby,
different T cell subsets can be identified as being related (derived
from a common progenitor within the naive T cell pool) if they share
genetic tags. Using this barcoding technology we have addressed
issues of cellular kinship and clonal diversity. With respect to the
first, we have demonstrated that antigen-specific effector and memory
CD8+ T cell populations are progeny of the same naive T cell
clones. With respect to the second, by using cellular barcoding to
measure the clonal diversity within T cell responses we have shown
that the efficiency by which antigen-specific naive T cells are
recruited into the immune response is remarkably constant for T cell
responses that are either weak or strong. Currently we are
investigating to what extent individual naive antigen-specific CD8+ T
cells contribute to the magnitude of the overall response. Do all T
cells of a given affinity for antigen produce equal numbers of
progeny, or is the total effector pool predominantly created by the
output of few? To quantify individual clone sizes, we have coupled our
barcoding technology to a deep-sequencing-based quantitative readout
system and found that after Listeria monocytogenes infection,
individual naive T cells produce highly variable numbers of daughter
cells, in spite of the fact that these T cells recognize the model
antigen with equal affinity.
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10:30
David McDowell (LGC Genomics)
DNA Sequencing in a Commercial Setting
The early history of DNA can be traced back to the late 19th century.
From these exciting yet modest beginnings and the now famous
experiments on inherited traits in peas by the Czeck monk Gregor
Mendel, the 21st century is rapidly proving to be the era when genomic
sequencing becomes routine.
So called next generation sequencing has a multitude of variations and
applications ranging from the analysis of specific gene regions to
whole genomes and transcriptomes. Such forms of sequence analysis
have migrated from academic labs through core facilities and into
commercial service laboratories where they are now part of the routine
service offering.
Commercial operations have much to offer both the industrial and
academic sectors in the current economic climate. A brief history of
DNA from discovery to the wide range of related services that can be
obtained from the modern genomics service laboratory will be
presented.
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11:30-12:00 coffee
- 12:00
Gareth Howell (Leeds)
Flow cytometry: introduction and applications of flow cytometry in biological research
An introduction to the technology and applications of flow cytometry,
from cell cycle to multiparameter immunophenotyping and high
throughput analysis and its potential for enabling computational
modeling of biological systems.
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13:00-14:00 lunch provided
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